RNA

Part:BBa_J100085:Experience

Designed by: Caroline Vrana   Group: Campbell M Lab   (2012-07-13)
Revision as of 15:06, 23 July 2012 by Cavrana (Talk | contribs) (→‎Applications of BBa_J100085)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J100085

This sequence is missing one nucleotide (T) from the GFP target spacer (part E0040). This nucleotide is number 683 in the E0040 sequence, and the T should be between 181 and 182 in part J100085 sequence. This caused the system to not destroy the plasmid containing GFP as it should. CRISPR short.jpg

  • CRISPR and GFP should have no green fluorescence

User Reviews

UNIQc2e0c1a85596b163-partinfo-00000000-QINU UNIQc2e0c1a85596b163-partinfo-00000001-QINU