Composite
Part:BBa_K727011:Design
Designed by: İBRAHİM YASİR ORHAN Group: iGEM12_AUC_Turkey (2012-06-16)
Fish Odor Generator-Component Three
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1053
Illegal suffix found in sequence at 2447
Illegal EcoRI site found at 2476
Illegal EcoRI site found at 3926
Illegal XbaI site found at 2491
Illegal XbaI site found at 3941
Illegal SpeI site found at 3898
Illegal SpeI site found at 5429
Illegal PstI site found at 3912
Illegal PstI site found at 5443 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1053
Illegal EcoRI site found at 2476
Illegal EcoRI site found at 3926
Illegal SpeI site found at 2448
Illegal SpeI site found at 3898
Illegal SpeI site found at 5429
Illegal PstI site found at 2462
Illegal PstI site found at 3912
Illegal PstI site found at 5443
Illegal NotI site found at 1059
Illegal NotI site found at 2455
Illegal NotI site found at 2482
Illegal NotI site found at 3905
Illegal NotI site found at 3932
Illegal NotI site found at 5436 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1053
Illegal EcoRI site found at 2476
Illegal EcoRI site found at 3926
Illegal BglII site found at 2428
Illegal BglII site found at 2600
Illegal BglII site found at 5295
Illegal BamHI site found at 4059 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1053
Illegal suffix found in sequence at 2448
Illegal EcoRI site found at 2476
Illegal EcoRI site found at 3926
Illegal XbaI site found at 2491
Illegal XbaI site found at 3941
Illegal SpeI site found at 3898
Illegal SpeI site found at 5429
Illegal PstI site found at 3912
Illegal PstI site found at 5443 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1053
Illegal EcoRI site found at 2476
Illegal EcoRI site found at 3926
Illegal XbaI site found at 1068
Illegal XbaI site found at 2491
Illegal XbaI site found at 3941
Illegal SpeI site found at 2448
Illegal SpeI site found at 3898
Illegal SpeI site found at 5429
Illegal PstI site found at 2462
Illegal PstI site found at 3912
Illegal PstI site found at 5443
Illegal NgoMIV site found at 5004
Illegal AgeI site found at 1391
Illegal AgeI site found at 1466
Illegal AgeI site found at 2207
Illegal AgeI site found at 2861
Illegal AgeI site found at 2870
Illegal AgeI site found at 3621
Illegal AgeI site found at 4276 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 987
Design Notes
Optimized for E.coli
Source
Unknown