Regulatory
Part:BBa_J100065:Design
Designed by: Rebecca Evans Group: Campbell M Lab (2012-06-11)
Synthetic Riboswitch
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 193 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 194
Illegal PstI site found at 208
Illegal NotI site found at 7
Illegal NotI site found at 201 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 194 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 194
Illegal PstI site found at 208
Illegal AgeI site found at 122 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 187
Design Notes
None
Source
http://www.ncbi.nlm.nih.gov/pubmed?term=Synthetic%20Riboswitches%20That%20Induce%20Gene%20Expression%20in%20Diverse%20Bacterial%20Species