Regulatory
pD

Part:BBa_K896900:Design

Designed by: Jesse Wu   Group: iGEM12_NYMU_Taiwan   (2012-04-06)
Revision as of 03:41, 27 April 2012 by Blackrabbit (Talk | contribs) (→‎Design Notes)

pD and rbsD from ΦX174


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR primers (TM:55C): 

X174_pD_FP: gcttctag cattttgttcatggtagagattctct
X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga

Source

Bacteriophage ΦX174 RF DNA

References

Sorensen S. E. et al (1998) Identification of the in vivo promoters of bacteriophages S13 and phi X174 and measurement of their relative activities. Biochem Cell Biol. 76(4):625-36.