Tag
NLS

Part:BBa_J176008

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-09-29)
Revision as of 17:44, 9 October 2012 by Kahaynes (Talk | contribs) (→‎Usage and Biology)

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NLS

SV40 nuclear localization sequence


Usage and Biology

  • Include within the ORF of a protein that you need transport into the nucleus (e.g., a synthetic transcription factor)
  • Fluorophore-NLS fusions are handy for making glowing nuclei
  • Very effective in cultured human cells

PKKKRKV motif from the Simian Virus 40 Large T-antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. In our hands, the localization appears to be very efficient in U2OS and HEK293 cells (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aNLS