Protein_Domain
AmCyan

Part:BBa_J176022

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-09-29)
Revision as of 03:12, 9 December 2011 by Kahaynes (Talk | contribs) (→‎Usage and Biology)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

AmCyan

Human codon-optimized AmCyan1 from pAmCyan1-C1


Usage and Biology

  • Open reading frame only
  • Requires promoter and translation start (e.g., Kozak) for expression
  • Excitation = 458 nm, emission = 489 nm


Human codon-optimized Anemonia majano cyan fluorescent protein (AmCyan1) is a version of CFP (blue). In our hands it works very well in human U2OS osteosarcoma cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None