Coding

Part:BBa_M36280:Design

Designed by: Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso   Group: Stanford BIOE44 - S11   (2011-11-29)
Revision as of 22:48, 6 December 2011 by Juliaj27 (Talk | contribs) (→‎Design Notes)

Human Alcohol Dehydrogenase 1B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 367
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 367
    Illegal NgoMIV site found at 885
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been codon optimized for high expression in E. Coli. We added the histidine tag after the alcohol dehydrogenase sequence to allow for enzyme purification by nickel affinity chromatography.

Source

P00325 (ADH1B_HUMAN) provided by UniProt.

References