Part:BBa_J176001

flyPCD

Polycomb chromodomain from the Drosophila (fruit fly) Pc protein (a.a. 16-78).
Alias: KAH03

Usage and Biology

Drosophila PCD of Pc1 (orange) Crystal structure reported by Fischle et al. 2011. Grey = H3 histone tail; Ball and stick = trimethylated lysine 27

• Protein Data Bank ID 1PDQ
• Mammalian expression vector required
• Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
  

See the human variant BBa_J176000 for more details on the biology of the Polycomb chromodomain.

REFERENCES:

1. Fischle, W, Wang, Y, Jacobs, SA, Kim, Y, Allis, CD, Khorasanizadeh, S. (2003) Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains. Genes Dev. 17:1870-1881.
2. Min, J, Zhang, Y, Xu, RM. (2003) Structural basis for the specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27. Genes Dev. 17:1823-1828.
3. Haynes, KA, Silver, PA. (2011) Synthetic reversal of epigenetic silencing. J Biol Chem. 286:27176-27182.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]