Composite

Part:BBa_K568008

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-10-26)
Revision as of 10:33, 2 November 2011 by Anna2011 (Talk | contribs)

Red light sensor with GFP reporter

Red light sensor with GFP reporter. For more information and references check the red light sensor motherpart (BBa_K568000). However, the data obtained did not match our expectations. We tested its function in JT2. The test of the assay was repeated several times. Our results indicate that the part is independed of the different light conditions active.

Therefore, testing or even the design of this part should be revised.

Experimental testing

25.10.2011 GFP Assay over night under different lighting conditions Diagram.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4449


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Categories
Parameters
None