Coding
Part:BBa_K627017:Design
Designed by: iGEM11:Potsdam_Bioware Group: iGEM11_Potsdam_Bioware (2011-10-28)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 117
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 143
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 796
Design Notes
This biobrick was built by PCR using the following PCR primers: