Generator
thRS1-V

Part:BBa_K537003

Designed by: Gloria Hlongwane   Group: iGEM11_WITS-CSIR_SA   (2011-09-09)
Revision as of 19:28, 28 October 2011 by Marco Weinberg (Talk | contribs)

Theophylline Riboswitch 1-Venus

This part regulates the expression of venus in a theophylline-dependent fashion through an RNA aptamer specific to theophylline.

This theophylline riboswitch (type1) biobrick activates the expression of the Venus fluorescent protein in a theophylline-dependent fashion. It is composed of a theophylline sensitive riboswitch clone 8.1 (Topp and Gallivan JACS 2007; BBa_K249026 and BBa_K411001) fused upstream to an adjacent Venus coding region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



For the pdf version of this datasheet, click here

[edit]
Categories
//rna/riboswitch
Parameters
None