Regulatory

Part:BBa_K553023:Design

Designed by: Giulio Bernardinelli   Group: iGEM11_UNITS_Trieste   (2011-10-25)
Revision as of 07:47, 28 October 2011 by Luca (Talk | contribs) (→‎Design Notes)

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Quorum sensing induced mammalian trascription factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K553023 encodes for a chimerical protein composed by a portion of the human p65 and the whole TraR. These two portions are joined by a NLS signal in order to allow the translocation of the protein into the necleus. BBa_K553023 associated with BBa_K553021 and BBa_K553022 is able to regulate the expression of specific genes under the stimulation of OXOC8. BBa_K553023 has been also codon optimized for the expression in mammals.

Source

DNA Synthesis

References

1) Neddermann P, Gargioli C, Muraglia E, Sambucini S, Bonelli F, De Francesco R, Cortese R. A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR. EMBO Rep. 2003 Feb;4(2):159-65.

2) Deepak Chandran, Frank T Bergmann and Herbert M Sauro. "TinkerCell: modular CAD tool for synthetic biology". Journal of Biological Engineering 2009, 3:19 DOI: 10.1186/1754-1611-3-1