Composite
Part:BBa_K642007:Design
Designed by: Ian Roney, Matthew Orton Group: iGEM11_uOttawa (2011-10-24)
Constitutive Adh1 distal-Gal1 proximal promoter tagged with yeGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 89
Illegal BsaI.rc site found at 1379
Design Notes
The yeGFP part (BBa_K319039) from last year didn't have a stop codon so this tagged promoter should have a terminator sequence cloned downstream before using.
Source
The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal1 promoter. The Gal1 promoter is a commonly used promoter in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.