DNA
5xGal4

Part:BBa_J176019:Design

Designed by: Brady Laughlin   Group: Haynes Lab   (2011-09-29)
Revision as of 03:09, 16 October 2011 by Kahaynes (Talk | contribs) (→‎Source)

5xGal4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR-cloned from the pNEB plasmid, using the following primers:
Forward: 5'-CCTTTCTAGACGGAGTACTGTCCTCCGAGC
Reverse: 5'-AAGGCTGCAGCGGCCGCTACTAGTCGGAGGACAGTACTCCGCTC

These primers add a XbaI site upstream of the part and SpeI, NotI, and PstI sites downstream. The PCR amplicon was resolved on a gel and the largest band was cut out and purified (the target is repetitive, so special care was taken to ensure the largest product was isolated). The DNA was digested with XbaI/PstI, inserted into an empty V0120 vector, and verified by sequencing.

Source

Plasmid pNEB (new England Biolabs).

References