Coding

Part:BBa_K649300:Design

Designed by: Goshi Sugano   Group: iGEM11_Tokyo_Tech   (2011-09-19)
Revision as of 16:07, 15 October 2011 by Notothenia (Talk | contribs) (→‎References)

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Arginase (rocF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None. However urea-cycle or arginine-fermentation are regulated by ArgR. If we want to get urea, we should use ArgBox or ArgR-lacked strain.

Source

Bacillus subtilis 168 strain.

References

Stefan Schuster, "A general definition of metabolic pathways useful for systematic organization and analysis of complex metabolic networks" Nature Biotechnology , vol. 18, p326-32 (2000)