DNA
Part:BBa_K676011:Design
Designed by: Meng Li Group: iGEM11_UCL_London (2011-09-19)
Gyrase Binding Site from pSC101
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Performed PCR to clone out the GBS from the pSC101 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.
Source
pSC101 Plasmid DNA - 4580 to 4864 bp
References
Mark Oram, Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003) A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu, pSC101 and pBR322 Strong gyrase Sites; the role of DNA sequence in modulating gyrase supercoiling and biological activity; Molecular Microbiology 50 (1) 333-347