Part:BBa_K624047
Safety Flag
The iGEM Safety and Security Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety [AT] igem [DOT] org with any questions.
Reason: Listeriolysin and Invasin parts
If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2021 Safety Page for more information.
RBS(pmsp3) + LLO + RBS(pmsp3) + Inv + RBS(pmsp3) + ECFP
RBS(pmsp3) + LLO + RBS(pmsp3) + Inv + ECFP
Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.
Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.
All of the ribosome binding sites are from Pmsp3, the strongest promoter of AMB-1. (BBa_K624012)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1417
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2423
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3040
- 1000COMPATIBLE WITH RFC[1000]
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