Generator

Part:BBa_K624056

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)
Revision as of 12:23, 9 October 2011 by Phonesents (Talk | contribs)

pYMB essentials + RBS(Pmsp3) + minC

pYMB essentials + RBS(Pmsp3) + minC

pYMB (BBa_K624004) is a shuttle vector for manipulating and transforming gene into AMB-1.

The ribosome binding site is from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None