Composite

Part:BBa_K594015:Experience

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-05)
Revision as of 02:35, 6 October 2011 by DonQuixia (Talk | contribs)

iGEM11_OUC Experience

Applications of BBa_K381001

From the beginning, we use the linearized plasmid backbone pSB1C3 in the 2011 spring DNA distribution. But the standardization with them all end up with failures.

Then, we began to seek for a new plasmid backbone. We found the K381001 in pSB1C3. If we cut the pSB1C3 with EcoR I and Pst I, we could get pSB1C3 plasmid backbone for standardization.

User Reviews

UNIQ392db4a979144645-partinfo-00000000-QINU

•••••

DonQuixia

When we use the pSB1C3 for standardization, we found that the pSB1C3 have the right antibiotic resistance but a wrong DNA length.

UNIQ392db4a979144645-partinfo-00000002-QINU

The verification

OUC-China.Review.jpg

The pSB1C3 is supposed to be 2049bp, however, it appears around the marker 3000bp. We have the pSB1C3 in K381001 sequenced and found its total length is 2944bp, and its total sequence is as below,

OUC-China.ExpDNA.png

Through sequence analysis, we could find that the base in blue have a 99% homology with the right pSB1C3. It lacks a C and a A in the beginning. In the end, it has a 71 base homology with the right pSB1C3. The other sequence left do not accord with the right pSB1C3.