Coding
raiR

Part:BBa_K594008:Design

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-01)
Revision as of 00:38, 6 October 2011 by DonQuixia (Talk | contribs) (→‎Source)

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a luxR-type regulator from Rhizobium etli CFN42


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 248
    Illegal NgoMIV site found at 344
    Illegal NgoMIV site found at 508
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Rhizobium etli CFN42 is also a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in Rhizobium etli CFN42, and then found the specific sequence in NCBI and asked the Rhizobium etli CFN42 from Nanjing Agricultural University. The raiI/R system have no threats to biosafety.

Source

We got the Rhizobium etli CFN42 from Nanjing Agriculture University, and then cloned the raiR gene from Rhizobium etli CFN42 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.

References

1.VIOLA ROSEMEYER et al.luxI- and luxR-Homologous Genes of Rhizobium etli CNPAF512 Contribute to Synthesis of Autoinducer Molecules and Nodulation of Phaseolus vulgaris.JOURNAL OF BACTERIOLOGY,Feb. 1998, p. 815–821.
2.Anne Edwards,1 Marijke Frederix,1 Florence Wisniewski-Dye´,2 Jacob Jones,1 Angeles Zorreguieta,3 and J. Allan Downie1*.The cin and rai Quorum-Sensing Regulatory Systems in Rhizobium leguminosarum Are Coordinated by ExpR and CinS, a Small Regulatory Protein Coexpressed with CinI.JOURNAL OF BACTERIOLOGY, May 2009, p. 3059–3067
3.Juan E. Gonza´lez and Melanie M. Marketon. Quorum Sensing in Nitrogen-Fixing Rhizobia, MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2003,p. 574–592