Generator

Part:BBa_K624056

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)
Revision as of 11:20, 9 October 2011 by Peichinghuang (Talk | contribs)

pYMB essentials + RBS(Pmsp3) + minC

pYMB essentials + RBS(Pmsp3) + minC

pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 minC,the division inhibitor,its overexpression would lead to septation inhibition at all potential division sites, a circumstance of filamentation. The ribosome binding site is from Pmsp3.(BBa_K624012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None