Reporter

Part:BBa_K543012

Designed by: Naoto Shimada   Group: iGEM11_Tokyo_Metropolitan   (2011-09-29)
Revision as of 18:35, 5 October 2011 by Naoto (Talk | contribs)

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plux-GFP (AHL inducible)

This device is useful for detective of AHL(3OC6HSL). Under existence of AHL, GFP is expressed.

BBa K543012.jpg

Qualitative　assay
Our experience is that inoculated E.coli (induced K543012) onto a plate. Then put filter paper on center of plate and spot 20ul of culture AHL producing E.coli in. After 37℃ incubation, check florescence of GFP.
Figure is result of assay. GFP is expressed around of filter paper. This result suggest that K543012 is work appropriately.
K543012 is twin with J55000 and I733005, but both of them are only planning, haven’t experienced yet. Our result is the first data of this device is work.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1713


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Categories
Parameters
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