Translational_Unit

Part:BBa_K557001

Designed by: Yinghong Lan   Group: iGEM11_USTC-China   (2011-10-02)
Revision as of 16:01, 5 October 2011 by Pythonwawa (Talk | contribs) (Background)

Aptamer-CheZ: theophylline-sensitive synthetic riboswitch+phosphatase which dephosphorylates CheY-P


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Background

Chemotaxis has always been a powerful tool in organizing bacteria’s movement and it’s always an important ability to reprogram bacteria to follow entirely new chemical signals. Riboswitches, which have been a hot issue of synthetic biology recently, can be used to guide E.Coli toward and precisely localized to a completely new chemical signal. We take advantage of them in [http://2011.igem.org/Team:USTC-China/Project our system] to act as the main power to drive two parts of a bacterial colony away from each other.

Acknowledgements

Here we want really want to thank the Professor J. S. Parkinson for providing ΔcheZ strain RP1616 and the corresponding wild type RP437 and Professor J. P. Gallivan for directing our construction of riboswitches. Aptamer-toggleswitch.PNG

[edit]
Categories
Parameters
ligandsTheophylline