Coding

Part:BBa_K624033

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)
Revision as of 22:23, 5 October 2011 by Phonesents (Talk | contribs)

minC cell division inhibitor (revised)

This part, minC, is known as a cell division inhibitor. Its first record on iGEM was made by 2006 Chiba iGEM team(BBa_J29040), but there is not an available part. 2010 Warsaw iGEM team submitted another minC (BBa_K299806) with different sequence. However, there was a PstI site in the middle of the sequence.

We have cloned the minC gene from E. coli K-12 strain MG1655 by performing PCR, with a single nucleotide synonymous mutation to eliminate the PstI site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aminC cell division inhibitor (revised)