Part:BBa_K117002:Experience

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Applications of BBa_K117002

This promoter is activated indirectly by AI-2 to promote whatever downstream gene ligated behind it.

Characterisation:

For information on characterisation of this new part, please visit Part K117010 Experience and Part K117008 Experience

User Reviews

UNIQ24b099bff808408f-partinfo-00000000-QINU UNIQ24b099bff808408f-partinfo-00000001-QINU

• Tokyo Tech 2011

Fluorescence intensity of promoter lsrA-gfp((BBa_K117002)-gfp) was almost the same as promoterless-gfp(negative control). Strain used in this assay lacks lsrR. This work is done by Takuya Tsubaki. This part(BBa_K117002) does not work properly. To confirm this, we introduced a gfp gene(BBa_J54103) downstream of the promoter. As a consequence, fluorescence intensity of lsrA promoter-gfp((BBa_K117002)-gfp) was almost the same as promoterless-gfp(negative control), showing that lsrA promoter(BBa_K117002) does not work properly. We improved this part.Our new lsrA promoter(BBa_K649100) works. If you want to know why our part works, click [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. here] and visit our team's wiki. [sample] Ptet-gfp on pSB1A2(JD22597) Promoterless-gfp on pSB6A1(JD22597) PlsrA-gfp on pSB1A2(BBa_K649104)(JD22597) PlsrA-gfp on pSB1A2(BBa_K11702-gfp)(JD22597) JD22597 is a strain lacking lsrR. [Method] 1.Overnight cultures of reporter strains grown at 37 °C in LB medium containing appropriate antibiotics were diluted 1:100 into 3 ml of LB medium and were incubated at 37 °C as fresh cultures. 2. After their OD590 reached 0.15, the fresh cultures were diluted 1:100. 3. After 4-hour incubation at 37 °C, 1 ml of each culture was moved to 1.6ml tube and its fluorescence intensity was measured with a flow cytometer.

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