RNA
Part:BBa_K581000:Design
Designed by: Chen Yiwei Group: iGEM11_Peking_S (2011-09-17)
sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is the G178C mutant of the small RNA SgrS(wt).
Teppei Morita et.al’ s work suggests that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. Since a specific repression effect is favored in our comparator device, the characteristics of the conjugate mutated ptsG/SgrS system meet the need quite well.
Also, such a site mutation is designed for biological orthogonality.
Source
E.coli K-12 strain.