Regulatory
pT7

Part:BBa_I719005:Experience

Designed by: Imperial 2007   Group: iGEM07_Imperial   (2007-10-24)
Revision as of 13:51, 19 October 2020 by 796935 (Talk | contribs) (→‎Applications of BBa_I719005)

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Applications of BBa_I719005

SUSTech_Shenzhen

Protein anti-CRISPR-associated 1 (Aca1) is expressed by T7 promoter, Aca1 will subsequently inhibit the anti-CRISPR promoter, so the gene expression downstream anti-CRISPR will be inhibited until Navitoclax (a compound that can inhibit Aca1 found by SUSTech_Shenzhen) is added artificially. Without the inhibition from Aca1, the expression of genes downstream anti-CRISPR promoter will increase significantly. Therefore, protein expression can be regulated artificially.

T7 promoter successfully worked in our composite part BBa_K3423005.

User Reviews

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Red_Dave

Macquarie_Australia (2011) used this T7 promoter to successfully express hemeoxygenase and create part BBa_K646003.

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