Coding

Part:BBa_K649102

Designed by: Hiroki Yoshise   Group: iGEM11_Tokyo_Tech   (2011-09-23)
Revision as of 14:10, 5 October 2011 by Yosei (Talk | contribs)

RBS-lsrR-lsrK

LsrR is the repressor of the lsr operon and itself.


LsrK is the kinase. LsrK phosphorylates AI2 and Phospho-AI2 relieves LsrR repression.


In BBa_K649101, lsrK has flame shift mutation. Therefore we made this part containing correct lsrK.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None