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Part:BBa_K649202

Designed by: SeoHyun Kim   Group: iGEM11_Tokyo_Tech   (2011-09-18)
Revision as of 23:43, 3 October 2011 by Notothenia (Talk | contribs)

PlacIQ-lox71-rfp-lox66-gfp

The lox sequences, lox71 and lox66, have 5 bp on the 5 and 3 ends changed, respectively. DNA segment flanked by lox71 and lox66 marks the point which the enzyme Cre will excise. This Part is expected to express GFP when the lox sites are excised and RFP when they are not. The Cre-mediated recombination of this BioBrick had been studied and proved to be working.

Effect of Cre recombinase induced by arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1016
    Illegal BamHI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 686
    Illegal AgeI site found at 798
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1700


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Parameters
n/aPlacIQ-lox71-rfp-lox66-gfp