Generator
Part:BBa_K649201
Designed by: SeoHyun Kim Group: iGEM11_Tokyo_Tech (2011-09-18)
Revision as of 23:33, 3 October 2011 by Notothenia (Talk | contribs)
PlacIQ-lox2272-rfp-lox2272-gfp
lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1016
Illegal BamHI site found at 14 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 686
Illegal AgeI site found at 798 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1700
[edit]
Categories
Parameters
n/a | PlacIQ-lox2272-rfp-lox2272-gfp |