Generator
Part:BBa_K524004:Design
Designed by: HO Yuan Heng Trevor Group: iGEM11_HKUST-Hong_Kong (2011-10-03)
pir gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 438
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Homologous sites in primers designed by researchers to clone the pir gene into the E. coli BW25141 genome were used to clone out and standardize the pir gene. This is to avoid truncation of pir gene, and therefore junk sequences of 154 base pairs can be found downstream of the pir CDS.
Source
Cloned out and standardized from the genome of strain BW25141 (courtesy of The Coli Genetic Stock Center).