Generator

Part:BBa_K649301

Designed by: Goshi Sugano   Group: iGEM11_Tokyo_Tech   (2011-09-19)
Revision as of 18:32, 3 October 2011 by Natsuki (Talk | contribs)

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.

introduction of rocF(arginase coding gene) led to production of some urea.
This work was done by Natsuki Kubo and GoshiSugano.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None