Signalling

Part:BBa_K574001:Design

Designed by: Lei Wei   Group: iGEM11_Tsinghua-A   (2011-09-29)
Revision as of 15:07, 3 October 2011 by Destiny (Talk | contribs) (→‎Design Notes)

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TetR regulated by 3OC6HSL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


Design Notes

To design a system with an output of TetR in response to detecting the 3OC6HSL, we construct this part. TetR can be an activater of the next circuit.

Source

We used BioBrick Standard Assembly. List as follows: BBa_K145270 BBa_R0062 BBa_P0440

References