Part:BBa_K117002:Experience
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K117002
This promoter is activated indirectly by AI-2 to promote whatever downstream gene ligated behind it.
Characterisation:
For information on characterisation of this new part, please visit Part K117010 Experience and Part K117008 Experience
User Reviews
UNIQf568d6176e5bf244-partinfo-00000000-QINU UNIQf568d6176e5bf244-partinfo-00000001-QINU
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Tokyo Tech 2011 |
This part(BBa_K117002) does not work properly. To confirm this, we introduced a gfp gene(BBa_J54103) downstream of the promoter. As a consequence, fluorescence intensity of promoter lsrA(BBa_K117002)-gfp was almost the same as promoterless-gfp(negative control), showing that promoter lsrA(BBa_K117002) does not work properly.
[sample] Ptet-gfp on pSB1A2(JD22597) Promoterless-gfp on pSB6A1(JD22597) PlsrA-gfp on pSB1A2(BBa_K649104)(JD22597) PlsrA-gfp on pSB1A2(BBa_K11702-GFP)(JD22597) [Method] 1.Overnight cultures of reporter strains grown at 37 °C in LB medium containing appropriate antibiotics were diluted 1:100 into 3 ml of LB medium and were incubated at 37 °C as fresh cultures. 2. After their OD590 reached 0.15, the fresh cultures were diluted 1:100. 3. After 4-hour incubation at 37 °C, 1 ml of each culture was moved to 1.6ml tube and its fluorescence intensity was measured with a flow cytometer.
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