Regulatory

Part:BBa_K578001

Designed by: iGEM11_Arizona_State   Group: iGEM11_Arizona_State   (2011-09-27)
Revision as of 21:59, 5 October 2011 by Ksstanda (Talk | contribs) (→‎Confirmation of Part Length)

E. coli CRISPR I leader sequence

BBa_K578001.

Usage and Biology

  • This is the AT-rich leader region of the CRISPR I array in Escherichia coli str. K-12 substr. MG1655. "Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS" (Ü. Pul et al, 2010) characterized a single promoter (Pcrispr1) in this sequence. Transcription is silenced through H-NS binding to the leader sequence. Induction requires anti-silencing, either by protein-independent processes, DNA-binding proteins or other modulatory mechanisms not yet identified.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Confirmation of Part Length

PCR of BBa_K578001 using Primers VF2 (BBa_G00100) and VFR (BBa_G00101) from pSB1C3. The part is 106bp plus 42bp for the Standard BioBrick prefix and suffix. Note: The primers add approximatellly 200bp to the BioBrick's size (The primary band visualized is larger than expected an requires sequencing confirmation).

ASU K12 Leader.png

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Categories
Parameters
None