Generator

Part:BBa_K624019

Designed by: Shang-Jui Tsai   Group: iGEM11_NYMU-Taipei   (2011-10-02)
Revision as of 12:52, 4 October 2011 by Sam255676 (Talk | contribs)

pYMB essentials+[13-R]+[Y-CHAMP]

pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, 13-R and Y-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. This design is made in case EYFP fusion on N-terminal of mms13 has negative effects on its folding. But according to the opposite location of EYFP on CHAMP, this part is intended for a negative control, also testing the orientation of CHAMP on the effect of BRET between EYFP and rLuc.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 1809
    Illegal NgoMIV site found at 1893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2559


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Categories
Parameters
None