Composite

Part:BBa_K609000:Design

Designed by: Ke Liu   Group: iGEM11_HIT-Harbin   (2011-10-01)
Revision as of 02:17, 6 October 2011 by Liu525 (Talk | contribs) (→‎Source)

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nisI+pH induced promoter rcfB+ repressor LacR of lac operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 841
    Illegal EcoRI site found at 886
    Illegal XbaI site found at 901
    Illegal SpeI site found at 1990
    Illegal PstI site found at 2004
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 886
    Illegal SpeI site found at 842
    Illegal SpeI site found at 1990
    Illegal PstI site found at 856
    Illegal PstI site found at 2004
    Illegal NotI site found at 15
    Illegal NotI site found at 849
    Illegal NotI site found at 892
    Illegal NotI site found at 1997
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 886
    Illegal BglII site found at 1099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 842
    Illegal EcoRI site found at 886
    Illegal XbaI site found at 901
    Illegal SpeI site found at 1990
    Illegal PstI site found at 2004
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 886
    Illegal XbaI site found at 24
    Illegal XbaI site found at 901
    Illegal SpeI site found at 842
    Illegal SpeI site found at 1990
    Illegal PstI site found at 856
    Illegal PstI site found at 2004
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is designed to let Lactobacillus bulgaricus stop producing lactic acid when the external pH declines to 5.5 or lower.


Source

The nisI gene comes from the Lactococcus lactis. The rcfB promoter is found in Lactococcus lactis IL1403, while the lacR gene is from Lactobacillus. delbrueckii subsp. lactis.

References