Coding
rhiI
Part:BBa_K594005:Design
Designed by: Yu Xiaoyan Group: iGEM11_OUC-China (2011-10-01)
a luxI homologous AHL synthetase from Rhzobium leguminosarum bv. vicae IMA57
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 394
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The rhi quorum sensing system show relationships with plasmid transfering. However, real transferring only occurs when these genes are combined with other essential genes like traABC, traI/R, so only rhi quorum sensing could not make the plasmid transfer. They will not arouse biosafety problems.
Source
it is from the Rhzobium leguminosarum bv. vicae IMA57 we purchased from the CGMCC(China General Microbiological Culture Collection Center).