Part:BBa_K549001:Experience

Our first experiment with this BioBrick showed positive results. Hypothetically the linear response for this system is fulfilled for a concentration range from 0.5 to 60 µM. But only to see, whether the BioBrick works or not, we started a pretest to see a yes-or-no-answer. For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output.

Transformed and induced E. coli cells, cultivated in LB-media (containing Ni(II)). left-hand side: phase contrast right-hand side: GFP-excitation;

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