DNA
5xGal4

Part:BBa_J176019:Design

Designed by: Brady Laughlin   Group: Haynes Lab   (2011-09-29)
Revision as of 02:59, 16 October 2011 by Kahaynes (Talk | contribs) (→‎Design Notes)

5xGal4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR-cloned from the pNEB plasmid, using the following primers:

Forward: 5'-
    CCTTTCTAGA
CGGAGTACTGTCCTCCGAGC
Reverse: 5'-
    AAGGCTGCAGCGGCCGCTACTAGT
CGGAGGACAGTACTCCGCTC

These primers add a XbaI site upstream of the part and SpeI, NotI, and PstI sites downstream. The PCR amplicon was digested with XbaI/PstI and inserted into an empty V0120 vector.

Source

TBA

References