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Part:BBa_K274004:Experience

Designed by: Shuna Gould   Group: iGEM09_Cambridge   (2009-10-19)
Revision as of 11:46, 29 September 2011 by Zcc (Talk | contribs) (→‎Characterisation by NCTU_FORMOSA)

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Applications of BBa_K274004

User Reviews

UNIQ50f5e7b81f9fdc32-partinfo-00000000-QINU UNIQ50f5e7b81f9fdc32-partinfo-00000001-QINU

E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.


Karina Arnesen, undergraduate, Alberta iGEM 2010

When digested with BamHI and NotI, the insert did not digest as expected, only a 2kb band (backbone) and ~6kb band were visible.

Characterisation by NCTU_FORMOSA

This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts.

We mutated the sequence to correct the Xba I restriction site .

And the sequence is made of vioABDE not vioABDE