Part:BBa_K656013

Sporosarcina pasteurii Urease

This genetic cassette represents the first modular, fully functional Sporosarcina pasteurii urease suite in the iGEM registry. It contains an 11kb gene cluster which encodes the multiple subunits and other genes necessary for the function of urease in Sporosarcina pasteurii. E. coli genetically transformed with this sequence has the ability to hydrolyze urea. Ureolytic activity of transformants has been confirmed using urease test plates, which change color as urea is hydrolyzed increases the pH of the medium. To see an image of urease plates changing color due to the activity of transoformants (streaked directly on the plate), please use the following link: http://2011.igem.org/File:Transformant_urease_plates.JPG.

The sequence of this cassette is currently unavailable because it currently does not exist in any known database, but we have begun to sequence it with the assistance of Dr. Chris Mason (Weill Cornell Medical College). As soon as the sequence data is available, we will supplement the directory listing.

Documentation of the BioBrick part: http://2011.igem.org/Team:Brown-Stanford/REGObricks/Biobrick

For information on qualitative and quantitative characterization of this part's function: http://2011.igem.org/Team:Brown-Stanford/REGObricks/Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]