Coding

Part:BBa_K632002

Designed by: Viki Ding   Group: iGEM11_Minnesota   (2011-09-28)
Revision as of 23:23, 9 October 2011 by Dingx055 (Talk | contribs)

INP-Silicatein

The silicatein gene has been successfully fused to an existent ice nucleation protein (BBa_K265009). Gel electrophoresis and DNA sequencing both suggest the fusion product is the correct size, frame, and orientation. Silicatein functional assay was performed to verify the activity of silicatein, and the results indicate the Silicatein is enzymatically active.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal SpeI site found at 2398
    Illegal PstI site found at 1433
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 1798
    Illegal SpeI site found at 2398
    Illegal PstI site found at 1433
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 1623
    Illegal BamHI site found at 337
    Illegal XhoI site found at 1387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal SpeI site found at 2398
    Illegal PstI site found at 1433
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal SpeI site found at 2398
    Illegal PstI site found at 1433
    Illegal NgoMIV site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None