Device
Part:BBa_K598026:Design
Designed by: Shuobing CHEN, Tong MU Group: iGEM11_Peking_R (2011-09-28)
AND gate (BBa_K228258) regulated by TPP riboswitch 1.20 (BBa_K598004)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1274
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2144
Illegal BamHI site found at 1214 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1976
Illegal AgeI site found at 1320 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1323
Design Notes
The RBS of the TPP ribozyme is the native one.
Source
The AND gate we utilized was designed by Anderson and his colleagues and consequently standardly redesigned by PKU_Beijing 09 team.