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Part:BBa_K648123:Design

Designed by: Elyse Merkel   Group: iGEM11_Penn_State   (2011-09-28)
Revision as of 02:58, 29 September 2011 by Ler172 (Talk | contribs) (→‎Design Notes)

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RecA with mutation of Lys 286 and Arg 243


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation of active RecA at two different amino acids (Arg 243 and Lys 286) to attempt to prevent RecA from repairing damaged DNA but not other functions

Source

RecA 1 DH10B BBa_648100

References