Coding

Part:BBa_K566028

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-27)
Revision as of 00:18, 29 September 2011 by Rhodwulf (Talk | contribs)

CcaR

CcaR coding sequence Ccar is the response regulator of CcaS, wich is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along CcaR, activates transcription of promoter pCpcG2. Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with the absorption of red light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal BamHI site found at 402
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None