Coding
cI434 opt
Part:BBa_K566011
Designed by: Daniel Rodriguez Group: iGEM11_UANL_Mty-Mexico (2011-09-26)
cI434 repressor from phage 434 optimized for E. coli cI (+LVA)
Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in E. coli.
Usage and Biology
Repressor protein cI from bacteriophage 434. cI434 has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM434 promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM434.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 436
Illegal AgeI site found at 447 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |