Composite

Part:BBa_K634007:Experience

Designed by: Eric Walters   Group: iGEM11_Wisconsin-Madison   (2011-09-23)
Revision as of 03:48, 28 September 2011 by Ewalters (Talk | contribs) (→‎Applications of BBa_K634007)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Issues in practice

sacB appears to be something of a lightning rod for loss of function mutations. As can be seen above, sucrose lethality is only maintained in a population for approximately 5 hours. At this point, we presume that spontaneous mutations in sacB produce sucrose-insensitive mutants.

User Reviews

UNIQb438fd8284b48f35-partinfo-00000000-QINU UNIQb438fd8284b48f35-partinfo-00000001-QINU