Composite

Part:BBa_K569010:Design

Designed by: David Golynskiy & Tyler Guinn   Group: iGEM11_UT_Dallas   (2011-09-27)
Revision as of 01:02, 29 September 2011 by Mtg082000 (Talk | contribs) (→‎Design Notes)

ctx+CheZ mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 818


Design Notes

 CTX
           foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
           reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'
 CheZ 
          5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' 
          5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3' 
          to add the BioBrick restriction enzymes sites.

Source

Na

References