Coding
cI opt
Part:BBa_K566012:Design
Designed by: Daniel Rodriguez Group: iGEM11_UANL_Mty-Mexico (2011-09-26)
cI repressor from Lambda phage optimized for E. coli cI (+LVA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 463
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Search for illegal sites for classic BioBricks was performed, they were detected and eliminated.
Source
Sequence was obtained form BioBrick BBa_C0052, it was optimized changing for preferential codons as possible and then synthesized.
References
1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.
2. Court DL, Oppenheim AB, Adhya LS (2007) A New Look at Bacteriophage λ Genetic Networks. J Bacteriol 189:298–304.